Hot start Taq DNA polymerase(500U)

FOR RESEARCH USE ONLY
Description:
Same outstanding polymerase as eDNA standard Taq
Polymerase activity inhibited by Taq antibody until initial denaturation temperature is reached
Ultrapure grade enzyme – all inhibitor/DNA contamination removed by affinity chromatography
 Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5?->3? polymerase activity and a double-strand specific 5?->3? exonuclease activity.
 Isolated from an E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus
 Performs well over a wide range of templates
 Incorporates dUTP, dITP and fluorescently-labeled nucleotides
 Supplied with 10X Reaction Buffer and 25mM MgCl2
 Applications:
 Routine PCR
 Real-Time PCR
 Primer extension
 Single-colony PCR
 Nick translation
 A-tailing of blunt dsDNA fragments for T-vector cloning
 End-labeling blund dsDNA fragments
 Enzyme Properties
 3? to 5? exonuclease activity: No
 5? to 3? exonuclease activity: Yes
 Strand Displacement: No
 Heat Inactivation: No
 Theoretical Molecular Weight: 94,000 daltons
 Error Rate: 285 x10-6 bases
 Reaction Conditions:
1X Standard Taq Reaction Buffer:
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP in 30 minutes at 75°C.
Unit Assay Conditions: 
1X Standard Taq Reaction Buffer, 200 ?M dNTPs including and 200 ?g/ml ssDNA template.2
Quality Analysis Assays
Absense of 3’to 5’ exonuclease activity
Absence of endonuclease activity
Absence of DNA contamination
Standard PCR positive and negative controls
Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
1mM PMSF
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% Igepal CA 630 
Storage Temperature: 
-20°C
Patent Info
Use of this product for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees
1. U.J. Desai and P.K. Pfaffle, Biotechniques 19, 780 (1995)
2. H. Tveit and T. Kristensen, Analytical Biochemistry 289, 96 (2001)