Azoxymethane

Source high-purity Azoxymethane (AOM), a specialized chemical reagent essential for oncology research and toxicological studies. As a potent colon-specific carcinogen, it is the gold standard for inducing colorectal cancer models in preclinical trials, enabling accurate evaluation of chemopreventive agents and therapeutic interventions. Ideal for pharmaceutical R&D laboratories and academic institutions. High-quality raw material. Get a quote today!

Product Specification

• Product Name: Azoxymethane (AOM)
• Appearance: Colorless to pale yellow liquid
• CAS No.: 25843-45-2
• Molecular Formula: C₂H₆N₂O
• Specification: Purity ≥98.0% (GC); Molecular Weight: 74.08 g/mol; Density: ~1.0 g/cm³; Boiling Point: 94–96°C; Water Content: <0.5%
• Ingredient Information: Potent alkylating agent; Carcinogenic and toxic; Soluble in water, ethanol, and organic solvents; Requires strict handling protocols and cold storage (-20°C)

Product Description

Azoxymethane (AOM) is a highly specific procarcinogen widely recognized in biomedical research for its ability to induce colorectal tumors in experimental animal models, particularly rodents. Upon metabolic activation in the liver, it converts into methylazoxymethanol (MAM), which alkylates DNA, leading to mutations primarily in the Apc and β-catenin genes. This mechanism closely mimics human sporadic colorectal cancer, making AOM an indispensable tool for studying carcinogenesis, tumor progression, and the efficacy of potential chemopreventive compounds. Its reliability in generating consistent tumor profiles ensures its continued demand in pharmaceutical development, toxicology screening, and basic cancer biology research.

Product Uses

This ingredient is exclusively used in preclinical research settings to establish rodent models of colorectal cancer. It is administered via intraperitoneal injection to mice or rats to induce aberrant crypt foci (ACF) and subsequent adenocarcinomas, facilitating the study of disease mechanisms. Researchers utilize AOM-induced models to evaluate the protective effects of dietary components, pharmaceutical drugs, and natural extracts against colon cancer. Additionally, it serves as a critical tool in toxicology studies to investigate DNA damage, repair mechanisms, and mutagenesis pathways. Due to its hazardous nature, it is strictly confined to controlled laboratory environments and is not used in consumer products or therapeutic applications.

Formulation Recommendation

Recommended Usage Level: For research purposes, typical dosing regimens involve weekly intraperitoneal injections of 10–15 mg/kg body weight for 6 weeks, or single high-dose protocols depending on the study design. Always refer to specific institutional animal care and use committee (IACUC) guidelines.
Addition Method: Prepare fresh solutions in sterile saline or phosphate-buffered saline (PBS) immediately before administration due to instability in aqueous solutions. Handle under a fume hood with appropriate personal protective equipment (PPE). Store stock solutions at -20°C in amber vials to prevent degradation and light exposure.
Compatibility: Compatible with standard physiological buffers for injection. Do not mix with strong oxidizing or reducing agents. Ensure complete dissolution in saline to prevent precipitation during injection. Stability is limited in solution; discard unused portions after 24 hours. Strictly adhere to safety data sheet (SDS) guidelines for disposal and handling due to carcinogenicity.