Aequorin from jellyfish (Aequorea sp.)
CAS NO.: 50934-79-7
Molecular Weight: 21.7 kDa
pI: 4.2 - 4.9
This product is soluble in water (10 mg/ml), yielding a clear colorless solution.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Application: Its blue light emission is used to measure Ca2+ concentration.
Aequorin is one of a group of photoproteins isolated from marine coelenterates which emit blue light in the presence of Ca2+ ions. This bioluminescence is somewhat unique in that molecular oxygen is not required for light emission. The chromophore, coelenterazine,1 is bound to aequorin and the binding of calcium triggers the oxidation of the chromophore, producing a photon of light with a wavelength of 470 nm. In vivo, many organisms have an associated green fluorescent protein which shifts the emission to higher wavelengths, making the color of the emitted light appear green.
Aequorin from Aequorea aequorea has been purified to homogeneity and has been sequenced from cDNA cloning into various hosts. It is an 189 amino acid protein formed into a hydrophobic core region where coelenterazine is bound as what is believed to be a peroxidized form, since molecular oxygen is not required for light emission. Crystallography studies of the protein have elucidated 3 possible binding sites for Ca2+, but only 1 of these is absolutely required for light output.
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