Rat Insulin(INS) ELISA Kit

Rat Insulin(INS) ELISA Kit

Purpose:
This kit allows for the determination of INS concentrations in Rat serum, blood plasma, cell culture supernatant and other biological fluids.
Principle of the Aassay:
The kit assay Rat INS level in the sample,use Purified Rat INS to coat microtiter plate wells, make solid-phase antibody, then add INS to wells, Combined INS which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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Disclaimer: the information on this website is from the internet for reference only. Please refer to the actual instructions attached to the product and the final interpretation is owned by the company.

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