Mouse IL-6 ELISA Kit
Assay range: 3pg/ml-120pg/ml
This kit allows for the determination of IL-6 concentrations in Mouse serum, cell culture supernates and other biological fluids.
Principle of Mouse IL-6 ELISA Kit
The kit assay Mouse IL-6 level in the sample, use Purified Mouse IL-6 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to wells, Combined IL-6 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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