Human E-Selectin ELISA Kit
This kit allows for the determination of E-Selectin concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the Assay:
The kit assay Human E-Selectin level in the sample，use Purified Human E-Selectin to coat microtiter plate wells, make solid-phase antibody, then add E-Selectin to wells, Combined E-Selectin which With HRP labeled , become antibody - antigen -enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E-Selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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