Chicken glucocorticoid, GC ELISA Kit

Chicken glucocorticoid, GC ELISA Kit

Description About Chicken glucocorticoid, GC ELISA Kit
This kit is used for in vitro quantitative detection of glucocorticoid (GC) content in chicken serum, plasma, tissue, cell supernatant and related liquid samples.
Detection principle: 
This kit uses the double antibody sandwich method to determine the level of chicken glucocorticoid (GC) in the specimen. Microporous plates were coated with purified chicken glucocorticoid (GC) antibody to make solid-phase antibodies, and glucocorticoid (GC) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled glucocorticoid (GC) antibody binding to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the glucocorticoid (GC) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of chicken glucocorticoid (GC) in the sample was calculated by a standard curve.

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