Human Blocking antibody,BA ELISA Kit

Human Blocking antibody,BA ELISA Kit

Product description:This immunoassay kit allows for the in vitro quantitative determination of human BA concentrations in cell culture supernates, serum, plasma and other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to BA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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