Pullulanase is begin in plants and microorganisms. Pullulanases from microorganisms, such as Bacillus brevis, Bacillus acidopullulyticus and Klebsiella sp., are commercially accessible as an automated enzyme preparation.

Pullulanase is a starch-debranching enzyme produced by the gram-negative bacillus Klebsiella pneumoniae. In this organism, the enzyme is aboriginal exported to the alien film and is afterwards appear into the advance medium. Evidence appear actuality indicates that pullulanase is a lipoprotein. It is allegedly actinic as a forerunner with a 19-residue-long arresting arrangement and adapted by the covalent adapter of palmitate to the cysteine balance which becomes the amino abuttals afterwards break of the arresting sequence.

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