Human nuclear factor-κB p65,NF-κB p65 ELISA Kit

Human nuclear factor-κB p65,NF-κB p65 ELISA Kit

Product description:This immunoassay kit allows for the in vitro quantitative determination of human NF-κB p65 concentrations in serum, plasma, tissue homogenates and other biological fluids.Test principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-κB p65. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-κB p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-κB p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of NF-κB p65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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